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Image Search Results
Journal: Biosensors & bioelectronics
Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
doi: 10.1016/j.bios.2018.04.002
Figure Lengend Snippet: Functional block diagram of the system. The platform includes: an excitation module (M1) consisting of LEDs (520 nm center) and excitation filters (520/40 nm); a sample chamber module (M2, that accommodates a microscope slide, nitrocellulose membrane, or cassette; an emission and signal readout module (M3), consisting of emission filters (605/70 nm), photodiodes, and charge integration readout electronics; a microcontroller module (M4), at the heart of sequence control, power, and data processing; a display and connectivity module (M5) which includes an optional mini display that prompts and presents results to the user. A smartphone with a user interface establishes data connectivity via Bluetooth, shows device status, and displays results with the capability of uploading the information for cloud-based processing and interpretation by a primary care physician.
Article Snippet:
Techniques: Functional Assay, Blocking Assay, Microscopy, Membrane, Sequencing, Control
Journal: Biosensors & bioelectronics
Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
doi: 10.1016/j.bios.2018.04.002
Figure Lengend Snippet: Potential improvement of colorimetric assays by using fluorescent labels. (A) Blue polystyrene/latex col-orimetry microspheres and fluorescent latex microspheres spotted on nitrocellulose. 10 µL of dilutions were spotted, and the number of microspheres at each dilution is shown. (B) Detection time for fluorescent latex dilutions spotted on nitrocellulose and glass analyzed by the low-cost array platform. Our platform can reliably detect 5×105 0.5 µm microspheres on nitrocellulose membranes. This represents 2 to 3 orders of magnitude decrease in the number of microspheres detected, when compared to the number of colored latex microspheres required for a visible (positive) test line. We predict that actual assay variables could limit this number to 2 orders of magnitude.
Article Snippet:
Techniques: